The present study was conducted in Betwa River near Mandideep Industrial Area extending from origin up to a stretch of 200 kms downstream to assess the presence of selective pesticides in the river water. Quarterly analysis was carried out during 2016-17 for water samples collected from surface and bottom waters at stations of Betwa River in Mandideep area. The study reveals the presence of few pesticides viz. Aldrin, Endosulphan and HCH, in traces at all the stations except its origin. Presence of Pesticide, though in low concentration suggests primary entry of it into river water which may be attributed to run offs from nearby agricultural fields. The present study emphasizes the need for alternative agricultural practices with application of organic bio-pesticides instead of depending heavily on chemical pesticides.