The final 4-thiazolidinone derivatives (4a-r) were synthesized from methyl acrylate/methmethyl acrylate and studied in vitro for antifungal activity against A. niger, C. albicans, A. flavus, T. rubrum, A. fumigatus and P. citrinium at the concentration of 40, 80 and 160 µg/ml. These compounds showed good antifungal activity. Compound 4a with p-chloro substitution at both the phenyl ring showed activity even better than the used standard drug, Voriconazole.