Journal of Chemistry

Validated liquid chromatographic method for the determination of tramadol in pharmaceutical dosage forms and human plasma

Author & Affiliation:
Anton Smith A
Department of Pharmacy, Annamalai University, Annamalainagar- 608002 Tamilnadu (INDIA)
Manavalan R.
Department of Pharmacy, Annamalai University, Annamalainagar- 608002 Tamilnadu (INDIA)
Kannan K.
Department of Pharmacy, Annamalai University, Annamalainagar- 608002 Tamilnadu (INDIA)
Rajendiran N.
Department of Pharmacy, Annamalai University, Annamalainagar- 608002 Tamilnadu (INDIA)
CAS in Marine Biology, Annamalai University, Parangipettai - 608 502 Tamilnadu (INDIA)
Keyword:
liquid chromatographic, determination of tramadol, pharmaceutical dosage
Issue Date:
June 2008
Abstract:

 

 

A simple and reliable liquid chromatographic method has been developed for the determination of tramadol HCl in the pharmaceutical preparation and biological fluids. The fluorescence of the product was found to have excitation at lmax 272 nm and emission at lmax 298 nm. The method shows high sensitivity with linearity range from 0.07 to 0.4 µg/ml. The lower limit of detection (LOD) was found to be 1.63 x 10-2 µg/ml, 4.79 x 10-1 µg/ml and limit of quantification (LOQ) was found to be 4.95 x 10-2 µg/ml, 1.45x 10-2 µg/ml in formulation and biological fluids respectively. The different experimental parameters affecting the fluorescence intensity were carefully studied and optimized. The proposed method was applied successfully for determination of tramadol in the pharmaceutical preparation and biological fluids. The percentage recovery was found to be 101.3347 ±0.7991; 101.196 ± 1.382 for formulation and biological fluids respectively.

 

Pages:
7-14
ISSN:
2319-8036 (Online) - 0973-3450 (Print)
Source:
DOI:
juc

Copy the following to cite this article:

A. S. A; M. R.; K. K.; R. N., "Validated liquid chromatographic method for the determination of tramadol in pharmaceutical dosage forms and human plasma", Journal of Ultra Chemistry, Volume 4, Issue 1, Page Number 7-14, 2018

Copy the following to cite this URL:

A. S. A; M. R.; K. K.; R. N., "Validated liquid chromatographic method for the determination of tramadol in pharmaceutical dosage forms and human plasma", Journal of Ultra Chemistry, Volume 4, Issue 1, Page Number 7-14, 2018

Available from: http://www.journalofchemistry.org/paper/751/

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