LC/ESI/MS/MS method development and validation for the determination of sulfonamide antibiotic residues in shrimp sample

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LC/ESI/MS/MS method development and validation for the determination of sulfonamide antibiotic residues in shrimp sample

Author & Affiliation:

B. SIDDA REDDY

Dept. of Chemistry, S. V. University, Tirupati-517 502, A.P. (India)

SUDHAKAR YADLAPALLI

First Source Laboratory Solutions LLP (Analytical), Hyderabad, T.S. (India)

Y. SUBBA RAO

DST-PURSE Centre, S. V. University, Tirupati- 517 502, A.P. (India)

P.R. PRASAD

Dept. of Chemistry, S. V. University, Tirupati-517 502, A.P. (India)

K. PRABHAKAR

Department of Chemistry, DKW Govt. Degree College, Nellore, A.P. (India)

N.Y. SREEDHAR

Dept. of Chemistry, S. V. University, Tirupati-517 502, A.P. (India)

Keyword:

sulfonamides, shrimp, liquid chromatography-mass spectrometry, method development, method validation

Issue Date:

January 2018

Abstract:

A liquid chromatography–tandem mass spectrometry (LC/ESI/MS/MS) method was developed and validated for the determination of ten sulfonamide antibiotic residues in raw shrimp meat: sulfadimethoxine, sulfachloropyridizine, sulfamethoxypyridizine, sulfamethazine, sulfamethizole, sulfamerazine, sulfathiazole, sulfamethoxazole, sulfadiazine, sulfapyridine. The procedure involves the extraction of homogenized sample into acetonitrile and diluted with mobile phase. The analysis was carried out using ODS-EP 5 micron, 100×2.1mm column and mobile phase consists of 0.1% formic acid (A) and acetonitrile (B) in gradient mode at a flow rate of 0.5 mL/min. The final extracts were analyzed by the sensitive and selective LC/ESI/MS/MS operating in positiveion multiple reactions monitoring (MRM) mode.

Pages:

26-36

ISSN:

2319-8036 (Online) - 0973-3450 (Print)

Source:

Vol.14 - No.1

PDF:

Click here to download full paper

DOI:

http://dx.doi.org/10.22147/juc/140105

Copy the following to cite this article:

B. S. Reddy; S. Yadlapalli; Y. S. Rao; P. Prasad; K. Prabhakar; N. Sreedhar, "LC/ESI/MS/MS method development and validation for the determination of sulfonamide antibiotic residues in shrimp sample", Journal of Ultra Chemistry, Volume 14, Issue 1, Page Number 26-36, 2018

Copy the following to cite this URL:

Available from: http://www.journalofchemistry.org/paper/579/

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