Antibacterial activity of plant Asparagus racemosus belonging to family LIliaceae ,was evaluated some selected human pathogenic microorganism (Pseudomonas aeroginosa,Escherichia coli, Staphylococcus aureus) following agar well diffusion method using different concentration (60%, 80%, 100%). Two solvents Methanol and Acetone were used for extraction. It was concluded from the results that Methanolic as well as Acetone root extract of Asparagus racemosus of were quite effective in inhibiting growth of staphylococcus aureus bacteria. Therefore, the root extract of this plant can be selected for further investigation to determine their therapeutic potential.