In (III) has been investigated with Citrulline and Hippuric acid as mixed-ligand complexes at dropping mercury electrode by the polarographic technique. The stability constants of In(III)-Citrulline and In(III)-Hippuric acid were determined first by the DeFord and Hume's method. Later the In(III) was studied with mixed-ligand Citrulline and Hippuric acid by the Schaaf and McMaster's method. The reduction of all mixed-ligand complexes has been found to be quasirreversible and diffusion controlled. Three species of mixed-ligand complexes MXY, MX2Y and MXY2 have been determined and found to be more stable than that of simple complexes. The detailed temperature effect has been also studied.
Bis-azo acid dyes were prepared by the coupling of 4,4'-methylene bis(anthranilic acid) with various pyrazolone coupling components. The resultant dyes were characterized by elemental analyses, IR and NMR spectral studies. The UV-visible spectral data have also been discussed in terms of structural property relationship. The dyeing assessment of all the dyes was evaluated on wool and nylon textile fibres. The results show that a better hue was obtained on wool and nylon fibers. The results of the anti-bacterial properties of the bis-azo acid dyes revealed that the toxicity of these dyes against bacteria is fairly good.
Alpha amylase was isolated and characterized from Aspergillus niger using source soregum sample. The maximum activity 0.6237µmol/ml/min was obtained and Km value of enzyme was 5.8 x 10-5. The enzyme of Aspergillus niger could produce alpha amylase under solid state fermentation. The optimal conditions for maximal amylase production, includes pH 6.9, temperature 37oC and salt 10 Mm concentration. The enzyme activity was determined by DNS method. Using standard maltose curve.
Modified electrophoresis technique viz. solution electrophoresis has been employed for the study of the metal ligand equilibira in solution. The method is based upon the migration of metal ion under electric influence in electrophoretic tube with variation in pH of the black ground electrolyte (ionic strength 0.1). The plot of pH Vs absorbance difference reveals the nature of complexation and help in calculating its equilibrium constants. The present work report the result on the binary viz. Cu(II), Ni(II), Co(II) and Zn(II)-Glycyl Glycine complexing system.
The twenty one systems of Er(III) with O, N, and S containing ligands have been characterized on the basis of various thermodynamic parameters viz; oscillator strength of transition (P), work function (A), partitions function (Q), thermodynamic efficiency of transition (TET) & ratio of partition function (rp). The study provides useful information about M-L interactions.
Analytical applications of 5-methylfuran-2-carbaxaldehyde thiosemicarbazone (5-MFAT) and 3-methylthiophene-2-carbaxaldehyde thiosemicarbazone (3-MTAT) are reported for the first time. The reagents have been synthesized and characterized using IR, and NMR spectral data. 5-MFAT and 3-MTAT have been used for the spectrophotometric determination of mercury(II). The reagents react with mercury(II) in basic medium (pH 8.0, ammonium chloride and ammonium buffer) to form yellow colored complex 1:2 (M: L) complexes. The color reactions are instantaneous and absorbance values remain constant for 12 hours. The molar absorptivity and sandell's sensitivity of 5-MFAT and 3-MTAT methods are found to be 2.5 x 104 and 1.87 x 104 L mol-1 and cm-1 and 0.008 and 0.0107 ìg cm-2 of mercury(II) respectively. The methods were successfully applied to a number of water (potable and polluted), biological and soil samples containing mercury(II). The results of the proposed method are in agreement with flame atomic absorption spectrophotometry. The methods have high accuracy and precision (s = 0.018 and 0.0042 for 4 ìg/ml in 5-MFAT and 3-MTAT methods respectively).
Formation constant of Er (III)-Systems with different sulphonanilide ligands have been measured with the help of spectrophotometeric method. Results indicate that isolation of these complexes in solid state difficult.
The solution studies of binary complex of Rifampicin with Ce(III), Dy(III) ions have been performed. The protonation constant of Rifampicin and the stability constant of complexes with Ce(III), Dy(III) ions at 303K were investigated. The ionic strength 0.01M is constantly maintained by adding NaClO4. The pK and logK values of the complexes were determined in 10% to 50% Dioxane Water medium by the pH metric method. The pK and logK values of the complexes increased as dioxane content increased.
N-phenyl-5-substituted aryl-3-p-(fluorophenyl) pyrazoles have been synthesized from cyclization of 4-fluoroacetophenone (1) with various benzaldehydes (2) to give 4fluorophenylsterylketone (3) followed by treatment with phenyl hydrazine. The title compounds and their derivatives have been characterized by their elemental and spectral analysis. The newly synthesized compounds are screened for anti-bacterial activity. A few of the compounds gave promising results.
A new thermally stable three component inorganic ion exchanger stannic phosphosilicate have been synthesized and the sample with maximum ion exchange capacity and almost total regeneration has been chosen for further studies. The product has been characterized by IR and TGA Studies. The chemical composition (sn : P: Si = 2:4:1), chemical stability, distribution coefficient for 10 metal ions, change in exchange capacity with ionic radii, effect of temperature on ion exchange capacity and effect of electrolyte concentration on distribution coefficients for certain metal ions have been studied. The quantitative separations like Pb (II) from Hg (II), Mg(II), Ni (II) and Zn(II) and Hg (II) from Cu (II), Cd (II) and Ni (II) were carried out. The recovery ranged from 94 to 100% with a variation of 2% for repetitive determinations. A few ternary separations such as Hg (II) Zn (II) Pb (II), Hg (II) Ni (II) Pb (II) and Hg (II) Cu (II) Pb (II) were also achieved on tin (IV) phosphosilicate columns.